Joint calls

Proteomics analysis of endosomal compartments in Arabidopsis

  • Acronym PRECIAR
  • Duration 1 January 2007 - 1 January 2010
  • Project leader Gerd Jürgens, University of Tübingen, Germany
  • Other project participants Kathryn Lilley, University of Cambridge, UK
    Sacco de Vries, Wageningen University, The Netherlands
    David Robinson, University of Heidelberg, Germany
    Fernando Anciento, University of Valencia, Spain
    Enrique Rojo de la Viesca, National Institute of Agricultural and Environmental Research and Technology INIA, Spain
  • Funding The German Research Foundation (DFG), Germany
    Ministry of Science and Innovation (MICINN) Spain
    Netherlands Genomics Initiative / Netherlands Organisation for Scientific Research (NGI/NWO), The Netherlands
    Biotechnological and Biological Sciences Research Council (BBSRC), UK
  • Total Granted budget € 939,059

Abstract

This proposal addresses "other topics that contribute to the development of the European Research Area in plant genomics" (Sub Call A). Plant performance including yield stability largely depends on the interaction with the biotic and abiotic environment as well as on cell-cell communication within the plant itself. Nutrient uptake and signaling are mediated by plasma membrane-localised proteins such as transporters and receptors whose activity is determined by interaction with downstream effectors as well as their availability through endosomal trafficking and sorting. Endosomal sorting results either in protein degradation via targeting to the vacuole or in protein recycling to the plasma membrane. To provide a necessary foundation for functional studies of plant performance as specified in other topics of Sub Call A, this project aims to analyse endosomal compartments of Arabidopsis by proteomics. Specifically, resident and cargo proteins of immuno-isolated, ultrastructurally defined endosomal compartments will be identified by MS and subsequently analysed for endosomal localisation in transgenic plants expressing Myc-tagged or GFP-fusion versions of newly-identified proteins. The anticipated results will establish the role of endosomal compartments in plasma-membrane protein trafficking and enable distinction of early/sorting from recycling endosomes.

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