Integrating genetics and high throughput genomics to identify genes underlying tomato quantitative trait loci (QTL) for metabolites that influence fruit quality

Abstract

In the current project we will combine state of the art high throughput genomics technologies with quantitative genetic approaches for rapid identification of genes underlying tomato metabolite QTL. In concert with these approaches, we will identify the links between major regulators of ripening, e.g. RIN, CNR and their associated metabolite changes. The role of candidate genes will be verified using various assays including targeting induced local lesions in genomes (TILLING). The project will make an important contribution to fundamental knowledge about the molecular basis of fruit ripening. Furthermore, the project will build on and add value to current investment in national and transnational programmes (e.g. EUSOL) which are focused on harnessing natural variation. These approaches will improve product quality for the European consumer as well as providing the potential to increase the prosperity of the European Bio-economy. The consortium consists of internationally recognized groups from universities and research institutes in Europe and the USA as well as a global supplier of vegetable seeds, collectively ensuring a framework for innovative excellence.